Canine Leproid Granuloma in Mineiros City Mid-West Region of Brazil


  • Debora da Silva Freitas Ribeiro Departamento de Medicina Veterinária, Centro Universitário de Mineiros (UNIFIMES), Mineiros, GO, Brazil.
  • Jhosani Beatriz Bispo da Silva Departamento de Medicina Veterinária, Centro Universitário de Mineiros (UNIFIMES), Mineiros, GO, Brazil.
  • Tatiane Souza Saldanha Departamento de Medicina Veterinária, Centro Universitário de Mineiros (UNIFIMES), Mineiros, GO, Brazil.
  • Juliana Evangelista Bezerril Departamento de Medicina Veterinária, Centro Universitário de Mineiros (UNIFIMES), Mineiros, GO, Brazil.
  • Rodrigo Martins Ribeiro Departamento de Medicina Veterinária, Centro Universitário de Mineiros (UNIFIMES), Mineiros, GO, Brazil.



Background: Among the bacterial dermopathy the canine leproid granuloma (CLG) is a nodular pyogranulomatous disorder that affects the skin or subcutaneous tissue mainly in the dorsal face of ear pinna, head, and extremity of members caused by Mycobacterium spp. The pathogenicity is still not well clarified regarding the causative agent, which has not yet been completely typified, but phylogenetically, it is related to Mycobacterium tilburgii, M. simiae, and M. genavense, in Brazil, by the species M. murphy. The objective of this study is to report a case of canine leproid granuloma, through cytology and histopathology, and present the therapeutic procedures until the regression of cutaneous lesion.

Case: A 5-year-old Boxer breed, intac male weighing 32 kg, was assisted at the Veterinary Clinic of UNIFIMES, in Mineiros City, Mid-West Region of Brazil, GO, Brazil.  The animal had 4 nodules in the ears with evolution of 30 days, with no pruritus and without previous treatment. During the physical exam, the animal had normal physiological parameters. The cutaneous lesions were characterised by papules and alopecic nodules of firm to fibroelastic consistency, with progressive increase, located in the convex face of the ears. The fine needle aspiration puncture technique (FNAP) and histopathology for a definitive diagnosis was used, allowing the differentiation between inflammatory processes, infectious and neoplastic. Furthermore, blood was collected for hemogram and biochemical analysis for the assessment of renal and hepatic functions. In cytology, the stained blades by the Diff-quick stain in the microscopic exam had elevated cellularity, with several macrophages, and bacilliform structures in the negative image. Staining was also conducted by the Ziehl-Neelsen technique, which showed the presence of alcohol-acid-resistant bacilli (AARB) inside macrophages and in the centre of granuloma. In the animal’s follow up, a punch biopsy for histopathologic was conducted. A predominance of macrophages of epithelioid appearance, which were localised in the centre of nodulations, variable quantities de-agglomerates of degenerated neutrophils, was observed. In the periphery of the lesion intense infiltrate of lymphocytes and plasmocytes was found. Based on the clinical history, in the physical exam and laboratory findings, a treatment for mycobacteriosis with oral enrofloxacin 10 mg/kg every 24 h associated to doxycycline 10 mg/kg every 24 h and rifampicin topical twice a day in the cutaneous lesions was initiated. After three months of treatment, the animal did not have collateral effects with the association of antibiotics and had a complete clinical resolution, without recurrence.

Discussion: Despite that the CLG etiopathogeny is not well clarified, it is important to highlight the involvement of insects, such as flies and mosquitoes, inoculating the mycobacteria. Despite the fact that large size dogs, of breeds with short hair that are raised outdoors, have greater susceptibility to CLG and the lesions can be located specially in the face and ears, it is recommended to use complementary exams, such as cytology and histopathology, to obtain a definitive diagnosis. The CLG is a disease already reported in some regions of the Brazilian territory; however, it is believed that it is underdiagnosed, making it difficult to effectively use the therapeutic protocol.


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Albanese F. 2017. Cytology of Canine and Feline Non-neoplastic Skin Diseases. In: Canine and Feline Skin Cytology: A Comprehensive and Illustrated Guide to the Interpretation of Skin Lesions via Cytological Examination. New York: Springer, pp.201-209.

Almeida M.B., Priebe A.P.S., Fernandes J.I., Yamasak E.M. & França T.N. 2013. Granuloma lepróide canino na região da amazônica - relato de caso. Arquivo brasileiro de Medicina Veterinária e Zootecnia. 65(3): 645-648.

Camelo Junior F.A.A., Alves C.C., Fonseca M.G.M., Soares M.A., Bilhalva M.A. & Brito R.S.A.B. 2019. Síndrome do granuloma lepróide em um cão na cidade de Pelotas: Relato de caso. Pubvet. 13(3): 1-4.

Carvalho F.C.G., Rosas T.M., Machado M.A., Lopes N.L., Loures F.H., Conceição L.G. & Fernandes J.I. 2017. Associação de enrofloxacino a doxiciclina no tratamento do granuloma lepróide canino: relato de caso. Brazilian Journal of Veterinary Medicine. 39(3): 203-207.

Conceição L.G., Acha L.M., Borges A.S., Assis F.G., Loures F.H. & Silva F. 2011. Epidemiology, clinical signs, histopathology and molecular characterization of canine leproid granuloma: a retrospective study of cases from Brazil. Veterinary Dermatology. 22(3): 249-256.

Graça R.F. 2007. Citologia para clínicos: como utilizar esta ferramenta diagnóstica. Acta Scientiae Veterinariae. 35(2): 267-269.

Greene C.E. & Gunn-Moore D.A. 2015. Infecções micobacterianas. In: Greene C.E. (Ed). Doenças Infecciosas em Cães e Gatos. 4.ed. Rio de Janeiro: Guanabara Koogan, pp.522-549.

Larsson C.E. & Maruyama S. 2008. Micobacterioses. Revista Clínica Veterinária. 72: 36-44.

Larsson C.E., Michalany N.S. & Pinheiro S.R. 1994. Mycobacteriosis in domestic dogs - Report of two cases. Revista Faculdade Medicina Veterinária Zootecnia USP. 31: 35-41.

Malik R., Martin P., Wigney D., Swan D., Sattler P. S., Cibilic D. & Hughes M.S. 2001. Treatment of canine leproid granuloma syndrome: preliminary findings in seven dogs. Australian Veterinary Journal. 79(1): 30-36.

Maruyama S., Brandão P.E., Castro A.M.M.G., Michalany N.S., Fyfe J., Malik R. & Larsson C.E. 2015. Diagnóstico molecular do granuloma lepróide canino, a partir de cortes histológicos emblocados em parafina, pela técnica de reação em cadeia de polimerase (PCR) - estudo retrospectivo (2002-2009). Ciências Agrárias. 36(5): 3129-3138.

Pereira M.A.A., Nowosh V., Suffys P.N., Queiroz G.B., Silva K.M.O., Lourenço M.C.S., Vicente A.C.P., Fontes A.N.B., Morgado S. & Neves R.C.S.M. 2018. PCR-based identification of Mycobacterium murphy causing Canine Leproid Granuloma Syndrome in Niterói, southeast Brazil case report. Arquivo Brasileiro Medicina Veterinária Zootecnia. 70(6): 1699-1702.

Timm K., Welle M., Friedel U., Gunn-Moore D. & Peterhans S. 2019. Mycobacterium nebraskense infection in a dog in Switzerland with disseminated skin lesions. Veterinary Dermatology. 30(3): 262-e80.

Wurster F., Bassuino D.M., Silva G.S., Oliveira Filho J.P., Borges A.S., Pavarini S.P., Drimeier D. & Luciana S.L. 2017. Granuloma lepróide canino: estudo de 27 casos. Pesquisa Veterinária Brasileira. 37(11): 1299-1306.



How to Cite

Ribeiro, D. da S. F., da Silva, J. B. B., Saldanha, T. S., Bezerril, J. E., & Ribeiro, R. M. (2021). Canine Leproid Granuloma in Mineiros City Mid-West Region of Brazil. Acta Scientiae Veterinariae, 49.