Hormonal Protocols for the Synchronization and Induction of Synchronized Estrus in Dairy Ewes Kept under Tropical Conditions

Higor Nascimento Silva Lombardo, Clara Ana Santos Monteiro, Karina Frensel Delgado, Aline Emerim Pinna, Carlos Otávio de Paula Vasconcelos, Luiz Altamiro Garcia Nogueira, Felipe Zandonadi Brandão, Mário Felipe Alvarez Balaro

Abstract


Background: Lacaune is an important sheep breed and shows reproductive seasonality which affects dairy product supply. The most common hormonal protocols are based on progesterone-releasing devices in order to reach reproductive activity. Due to international consumer demand for natural, green, and clean methods, such issues encourage the minimal or absent use of synthetic hormones in livestock. In this sense, the present study tested the efficiency of induction and estrus synchronization protocols in lacaune ewes, considering: application interval of synthetic prostaglandin at the breeding season; length of synthetic progesterone exposure; and eCG dose at the outbreeding season.

Materials, Methods & Results: In study 1, 52 ewes were submitted to estrus synchronization protocol using two doses of d-cloprostenol 7 or 9 days apart. In study 2, 47 ewes were submitted to estrus induction protocol using an intravaginal device containing 60 mg of medroxyprogesterone acetate maintained for 6, 9, or 12 days. In study 3, 46 ewes were submitted to synchronized estrus induction protocol using intravaginal progesterone device for six days. On the fifth day, d-cloprostenol and 300 IU or 400 IU of eCG were administered. For all studies, signs of estrus beginning/ending were recorded and natural mating was performed. After 30 days, transrectal ultrasound was performed for pregnancy diagnosis. In study 1, groups presented similar rate of estrus behavior (78.8%) and pregnancy (average 75.6%). Meanwhile, compared to G9days (37.8 ± 7.2 h), interval between the second dose of prostaglandin and beginning of estrus was smaller in G7days (31.5 ± 7.8 h). No difference was found among variables studied in study 2 (P > 0.05), nevertheless the SD for the estrus duration was shorter (P < 0.05) in G6days and G12 days when compared with the G9days. Equally, the SD for the interval between sponge withdrawal and beginning and end of estrus was shorter (P < 0.05) in G12days when compared with the G9days. The pregnancy rate, at 30 days, was 71.1%. With regards to study 3, there was no effect of parturition order on sexual behavior between experimental groups (P > 0.05). Equally, no differences were found between variables studied (P > 0.05). The pregnancy rate was 52.2% and number of concepts was 1.5 ± 0.6.

Discussion: This paper provides important data on estrus synchronization protocols in dairy sheep kept under tropical conditions. In study 1, both d-cloprostenol protocols were efficient in synchronizing estrus of dairy ewes and reached a fair pregnancy rate. Therefore, it was demonstrated that a d-cloprostenol treatment was a viable alternative to a progestogen treatment, commonly used for estrus synchronization in ewes even during the breeding season. In study 2, all protocols (6, 9 and 12 days of exposure to synthetic progesterone) were efficient in inducing and synchronizing estrus in dairy ewes during the outbreeding season. Therefore, considering not only its efficiency but also the reduction of damage to the vaginal microbiota, the authors strongly encourage the use of the 6-day synthetic progesterone exposure protocol. The third study showed that it is possible to reduce the eCG dose (400 to 300 IU) in dairy ewes while maintaining protocol efficiency, saving 25% of the farmer spending, and avoiding high doses that are related to immune resistance and lower progressive fertility response to IA. In conclusion, the present study verified the efficiency of different hormonal protocols applied for dairy Lacaune ewes kept under tropical conditions, demonstrating that it is possible to decrease: (i) the interval between prostaglandin applications for 7 days apart during the breeding season; (ii) the length of exposure to synthetic progesterone for 6 days; and (iii) the eCG dose to 300 IU during the outbreeding season.


Full Text:

PDF

References


Abecia J.A., Forcada F. & González-Bulnes A. 2012. Hormonal control of reproduction in small ruminants. Animal Reproduction Science. 130(3-4): 173-179.

Alavez Ramírez A., Arroyo Ledezma J., Montes Pérez R., Zamora Bustillos R., Navarrete Sierra L.F. & Magaña Sevilla H. 2014. Short communication: Estrus synchronization using progestogens or cloprostenol in tropical hair sheep. Tropical Animal Health and Production. 46(8): 1515-1518.

Barillet F., Marie C., Jacquin M., Lagriffoul G. & Astruc J. 2001. The French Lacaune dairy sheep breed: use in France and abroad in the last 40 years. Livestock Production Science. 71(1): 17-29.

de Almeida S.F.C.M, Souza-Fabjan J.M.G., Balaro M.F.A., Bragança G.M., Pinto P.H.N., de Almeida J.G., Moura A.B.B., da Fonseca J.F. & Brandão F.Z. 2018. Use of two doses of cloprostenol in different intervals for estrus synchronization in hair sheep under tropical conditions. Tropical Animal Health and Production. 50(2): 427-432.

Fierro S., Viñoles C. & Olivera-Muzante J. 2016. Concentrations of steroid hormones, estrous, ovarian and reproductive responses in sheep estrous synchronized with different prostaglandin-based protocols. Animal Reproduction Science. 167: 74-82.

Fleisch A., Bollwein H., Piechotta M. & Janett F. 2015. Reproductive performance of Lacaune dairy sheep exposed to artificial long days followed by natural photoperiod without and with additional progestagen treatment during the nonbreeding season. Theriogenology. 83(3): 320-325.

Hasani N., Ebrahimi M., Ghasemi-Panahi B. & HosseinKhani A. 2018. Evaluating reproductive performance of three estrus synchronization protocols in Ghezel ewes. Theriogenology. 122: 9-13.

Knights M. & Singh-Knights D. 2016. Use of controlled internal drug releasing (CIDR) devices to control reproduction in goats: A review. Animal Science Journal. 87(9): 1084-1089.

Manes J., Campero C., Hozbor F., Alberio R. & Ungerfeld R. 2015. Vaginal Histological Changes after Using Intravaginal Sponges for Oestrous Synchronization in Anoestrous Ewes. Reproduction in Domestic Animals. 50(2): 270-274.

Manes J., Ríos G., Fiorentino M.A. & Ungerfeld R. 2016. Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa. Theriogenology. 85(5): 856-861.

Martin G.B. & Kadokawa H. 2006. “Clean, Green and Ethical” Animal Production. Case Study: Reproductive Efficiency in Small Ruminants. Journal of Reproduction and Development. 52(1): 145-152.

Martinez-Ros P., Astiz S., Garcia-Rosello E., Rios-Abellan A. & Gonzalez-Bulnes A. 2018. Effects of short-term intravaginal progestagens on the onset and features of estrus, preovulatory LH surge and ovulation in sheep. Animal Reproduction Science. 197: 317-323.

Martinez-Ros P., Astiz S., Garcia-Rosello E., Rios-Abellan A. & Gonzalez-Bulnes A. 2019. Onset of estrus and preovulatory LH surge and ovulatory efficiency in sheep after short-term treatments with progestagen-sponges and progesterone-CIDRs. Reproduction in Domestic Animals. 54(2): 408-411.

Martinez-Ros P., Lozano M., Hernandez F., Tirado A., Rios-Abellan A., López-Mendoza M. & Gonzalez-Bulnes A. 2018. Intravaginal Device-Type and Treatment-Length for Ovine Estrus Synchronization Modify Vaginal Mucus and Microbiota and Affect Fertility. Animals. 8(12): 226.

Martinez-Ros P., Rios-Abellan A. & Gonzalez-Bulnes A. 2018. Influence of Progesterone-Treatment Length and eCG Administration on Appearance of Estrus Behavior, Ovulatory Success and Fertility in Sheep. Animals. 9(1): 9.

Martinez M.F., McLeod B., Tattersfield G., Smaill B., Quirke L.D. & Juengel J.L. 2015. Successful induction of oestrus, ovulation and pregnancy in adult ewes and ewe lambs out of the breeding season using a GnRH+progesterone oestrus synchronisation protocol. Animal Reproduction Science. 155: 28-35.

Rana J. & Paul J. 2017. Consumer behavior and purchase intention for organic food: A review and research agenda. Journal of Retailing and Consumer Services. 38: 157-165.

Roy F., Combes B., Vaiman D., Cribiu E.P., Pobel T., Delétang F., Combarnous Y., Guillou F. & Maurel M.C. 1999. Humoral Immune Response to Equine Chorionic Gonadotropin in Ewes: Association with Major Histocompatibility Complex and Interference with Subsequent Fertility. Biology of Reproduction. 61(1): 209-218.

Texeira T.A., da Fonseca J.F., de Souza-Fabjan J.M.G., de Rezende Carvalheira L., de Moura Fernandes D.A. & Brandão F.Z. 2016. Efficiency of different hormonal treatments for estrus synchronization in tropical Santa Inês sheep. Tropical Animal Health and Production. 48(3): 545-551.

Ungerfeld R. & Rubianes E. 2002. Short term primings with different progestogen intravaginal devices (MAP, FGA and CIDR) for eCG-estrous induction in anestrus ewes. Small Ruminant Research. 46(1): 63-66.

Viñoles C., Meikle A., Forsberg M. & Rubianes E. 1999. The effect of subluteal levels of exogenous progesterone on follicular dynamics and endocrine patterns during early luteal phase of the ewe. Theriogenology. 51(7): 1351-1361.




DOI: https://doi.org/10.22456/1679-9216.102644

Copyright (c) 2020 Mário Felipe Alvarez Balaro

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.