STABILITY-INDICATING METHOD FOR DETERMINATION OF TIZANIDINE HYDROCHLORIDE IN PHARMACEUTICAL LC-CAD METHOD USING CHEMOMETRIC APPROACH

Authors

  • Mariana Brandalise Universidade Federal do Rio Grande do Sul-UFRGS
  • Pamela Cristina Lukasewicz Ferreira Universidade Federal do Rio Grande do Sul-UFRGS
  • Andrea Garcia Pereira Universidade Federal do Rio Grande do Sul
  • Leonardo Meneghini Universidade Federal do Rio Grande do Sul-UFRGS
  • Pedro Eduardo Fröehlich Universidade Federal do Rio Grande do Sul-UFRGS
  • Ana Maria Bergold Universidade Federal do Rio Grande do Sul-UFRGS

DOI:

https://doi.org/10.22456/2527-2616.75607

Abstract

A new LC method for tizanidine hydrochloride in tablet dosage form using a charged aerosol detector (CAD) is described. The influence of various parameters on chromatographic system was investigated by factorial designs and Derringer's desirability. Chromatographic conditions were: mobile phase constituted of acetonitrile and ammonium acetate buffer 17 mM (60:40; v/v), column oven at 39 °C and flow rate 0.8 mL.min-1 performed in on Acclaim Trinity P1 column UV at 230 nm. Thus, it was possible to validate a simple method to assay the tizanidine and its counter-ion in three formulations (drug reference, generic and manipulated). Method showed specificity when challenged by forced degradation and excipients. Finally, the method was compared with USP monograph method demonstrating equivalence in assay evaluation.

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Published

2017-08-28

How to Cite

Brandalise, M., Lukasewicz Ferreira, P. C., Garcia Pereira, A., Meneghini, L., Fröehlich, P. E., & Bergold, A. M. (2017). STABILITY-INDICATING METHOD FOR DETERMINATION OF TIZANIDINE HYDROCHLORIDE IN PHARMACEUTICAL LC-CAD METHOD USING CHEMOMETRIC APPROACH. Drug Analytical Research, 1(1), p. 61–66. https://doi.org/10.22456/2527-2616.75607

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ORIGINAL ARTICLES