The Effects of Separate and Combined Use of PGF2α and GnRH Hormones and the Addition of Βeta-Carotene on Fertility Parameters in Dairy Cows with Ovarian Cysts

Authors

  • Birten Emre Department of Obstetrics and Gynecology (Dep. O & G), Faculty of Veterinary Medicine (FMV), Harran University, Sanliurfa, Turkey.
  • İbrahim Küçükaslan Dep. O & G, FVM, Dicle University, Diyarbakır, Turkey.
  • Ali Reha Ağaoğlu Dep. O & G, FVM, Mehmet Akif Ersoy University, Burdur, Turkey.
  • Serhan Serhat Ay Dep. O & G, FVM, Ondokuz Mayıs University, Samsun, Turkey.
  • Duygu Kaya Dep. O & G, FVM, Kafkas University, Kars, Turkey.
  • Mustafa Somali Süt Kardesler Süt ve Süt Urn, Izmir, Turkey.
  • Selim Aslan Dep. O & G, FVM, Near East University, Nicosia, Turkey.

DOI:

https://doi.org/10.22456/1679-9216.84205

Abstract

Background: Ovarian cysts are commonly observed pathologies, which interfere with normal cyclic activity and adversely affect fertility in cows. Beta-carotene is effective in the reduction of reproductive problems by inducing the natural defence mechanisms of the body. There are several methods that can be used for the treatment of ovarian cysts. The separate and combined use of GnRH and PGF2α commonly uses in the treatment of ovarian cysts. Therefore, in the presented study the effects of Beta-carotene (βC) addition for the treatment of ovarian cysts either with GnRH solely or GnRH and PGF2α in combination on the fertility parameters of dairy cows were investigated.

Materials, Methods & Results: Seventy-six Holstein Friesian cows having ovarian cysts diagnosed by ultrasonography (USG) were divided into three groups. Cows in Group I (GI, n = 27), were injected with GnRH (Buserelin acetate, 5 mL, im), PGF2α (Tiaprost-trometamol, 5 mL, im) and βC (20 mL/cow, into 4 regions by im route). In Group II (GII, n = 25) GnRH (Buserelin acetate, 5 mL, im) and PGF2α (Tiaprost-trometamol, 5mL, im) were administrated while GnRH (Buserelin acetate, 5 mL, im) solely in Group III (GIII, n = 24). Cysts were monitored via USG, and blood samples were collected on the on day of treatment (day 0) and on the 7th and 14th days following the administrations. Cows shoving oestrous were inseminated and pregnancy diagnoses were performed on the 40th day following insemination. Treatment results showed that there were statistically no significant differences between GI and GII (P > 0.05). Only numerical difference obtained in time from therapy to pregnancy and overall pregnancy index (P > 0.05). Overall pregnancy rate (85 %), first service pregnancy rates (40 %) and overall pregnancy index (2.11) in GI were found significantly higher than GIII (53.3 %; 20 %; 4.12) [P < 0.05]. No significant difference was observed in progesterone (P4) levels between the groups (P > 0.05). It was found that βC administrations significantly increased βC levels in GI than GII and GIII on the 7th and 14th days (P < 0.05).

Discussion: One of the most common problems encountered in modern dairy production is the development of ovarian cysts. Treatments for ovarian cyst are numerous and variable, and have changed considerably over the years. In the present study, GnRH and PGF2α were administered together as a combination, and as a result of this combined use, higher percentages were obtained for both pregnancy rate and fertility parameters in GI and GII, in comparison to the group administered with GnRH alone (GIII). Better outcome from combination therapy (GnRH and PGF2α) may be due to the fact that luteal thickening in the walls of cysts was determined by ultrasonography, but P4 values were not identified immediately and the treatments were not categorized in accord with these values. No statistically significant differences were determined between GI and GII concerning the fertility parameters investigated, however numerical and proportional differences were observed. βC levels were significantly higher on day 7 and 14 after treatment in GI which were administered βC additionally to the treatment protocol for ovarian cysts. This statistical difference suggests that administration of βC in combination therapy is also effective in the treatment of ovarian cysts. In conclusion, it was determined that high pregnancy rates were obtained by the combined treatment of ovarian cysts (GnRH + PGF2α) and number of inseminations per conception were at desired limits. Better percentile and numerical fertility parameters were achieved in the group, which additionally received βC, high numbers of infertility cases, βC supplementation could be a viable option for treatment.

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References

Arbeiter K., Aslan S., > Schwarzenberger F. 1990. Untersuchungen über die Ovarzyste beim Rind-Entstehung, Therapieerfolge, Fruchtbarkeit. Deutsche Tierarztliche Wochenschrift. 97: 380-382.

Aréchiga C.F., Staples C.R., McDowell L.R. > Hansen P.J. 1998. Effects of Timed Insemination and Supplemental β-Carotene on Reproduction and Milk Yield of Dairy Cows Under Heat Stress. Journal of Dairy Science. 81(2): 390-402.

Aslan S., Handler J. > Arbeıter K. 1998. Frühgravidität und embryonale bzw. Frühfetale Mortalität bei der Kuh –Gelbkörperdynamik, Progesteron, Vitamin E, Vitamin-B12, ß-Carotin und Folsäurekonzentrationen im peripheren Blut. Wiener Tierärztliche Monatsschrift. 85: 141-147.

Ay S.S., Kaya D., Kucukaslan I., Agaoglu A.R., Emre B., Handler J., Fındık M. > Aslan S. 2012. Beneficial effects of Beta-carotene injections prior to treatment with PGF2α on the fertility of postpartum dairy cows. Revue de Médecine Vétérinaire. 163(8-9): 387-392.

Ay S.S., Kucukaslan I., Kaya D., Mulazımoglu S.B., Emre B., Kacar C., Kalender H., Fındık M., Bollweın H., Rıegler M., Schäfer-Somı S., Scholbach J. > Aslan S. 2012. The Change in Luteal Blood Flow and Luteal Size after Beta Carotene and GnRH Injections in Early Pregnant Dairy Cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 18(6): 1035-1041.

Ayasan T. > Karakozak E. 2010. Hayvan Beslemede β-Karoten Kullanılması ve Etkileri. Revıew. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 16(4): 697-705.

Bartolome J.A., Archbold L.F. > Morresey P. 2000. Comparison of synchronization of ovulation and induction of oestrus as therapeutic strategies for bovine ovarian cysts in the dairy cows. Theriogenology. 53(3): 815-825.

Baştan A. > Gürbulak K. 2015. Metabolic Disorders. In: Semacan A., Kaymaz M., Fındık M., Rişvanlı A. & Köker A. (Eds.). Obstetric and gynecology in farm animal. Malatya: Medipres Turkey, pp.331-363.

Bierschwal C.J., Garverick H.A., Martin C.E., Youngquist R.S., Cantley T.C. > Brown M.D. 1975. Clinical response of dairy cows with ovarian cysts to GnRH. Journal of Animal Science. 41(6): 1660-1665.

Bindas E.M., Gwazdauskas F.C., McGillard M.L. > Polan C.E. 1984. Progesterone responses to Human Chorionic Gonadotropin in dairy cattle supplemented with β-carotene. Journal of Dairy Science. 67(12): 2978-2985.

Cattaneo L., Signorini M.L., Bertoli J., Bartolome J.A., Gareis N.C., Diaz P.U., Bo G.A. > Ortega H.H. 2014. Epidemiological Description of Cystic Ovarian Disease in Argentine Dairy Herds: Risk Factors and Effects on the Reproductive Performance of Lactating Cows. Reproduction in Domestic Animals. 49(6): 1028-1033.

Chew B.P. 1993. Role of carotenoids in the immune response. Journal of Dairy Science. 76: 2804-2811.

Çelik H.A., Avcı G., Aydın İ., Bülbül A. > Bülbül T. 2009. Effect of β-carotene on ovarium functions and ovsynch success in repeat breeder cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 15(1): 87-94.

Dovenski T., Trojacanec P., Kochoski L.J., Petkov V., Popovski K. > Trojacanec S. 2007. Efficiency of Beta-Carotene for treatment of ovarian disorders in dairy cows. Reproduction of Domestic Animals. 42: 116.

Garverick H.A. 1999. Ovarian follicular dynamics and endocrine profiles in cows with ovarian follicular cysts. In. Howard J.L. & Smith R.A. (Eds). Current Veterinary Therapy, Food Animal Practice. Philadelphia: WB Saunders Co., pp.577-580.

Gossen N. > Hoedemaker M. 2005. Effect of beta-carotin serum concentration on the reproductive performance in dairy cows. Berliner und Munchhener Tierarztliche Wochenschrift. 118(7-8): 326-33.

Graves-Hoagland R.L., Hoagland T.A. > Woody C.O. 1988. Effect of β carotene and vitamin A on progesterone production by bovine luteal cells. Journal of Dairy Science. 71(4): 1058-1062.

Gumen A. > Wiltbank M.C. 2002. An alteration in the hypothalamic action of estradiol due to lack of progesterone exposure can cause follicular cysts in cattle. Biology of Reproduction. 66: 1689-1695.

Gundling N., Drews S. > Hoedemaker M. 2015. Comparison of Two Different Programmes of Ovulation Synchronization in theTreatment of Ovarian Cysts in Dairy Cows. Reproduction of Domestic Animals. 50(6): 893-900.

Ikeda S., Kitagawa M., Imai H. > Yamada M. 2005. The roles of vitamin A for cytoplasmic maturation of bovine oocytes. Journal of Reproduction and Development. 51(1): 23-35.

Inaba T., Mezan M., Shimizu R., Nakano Y. > Mori J. 1986. Plasma Concentrations of β-Carotene and Vitamin A in Cows with Ovarian Cyst. The Japanese Journal of Veterinary Science. 48(6): 1275-1278.

Iwańska S., Lewicki C. > Rybicka M. 1985. The effect of beta carotene supplementation on the beta carotene and vitamin A levels of blood plasma and some fertility indices of dairy cows. Archiv Fur Tierernahrung. 35(8): 563-70.

Jou P., Buckrell B.C., Liptrap R.M. > Summerlee A.J.S. 1999. Evaluation of the effect of GnRH on follicular ovarian cysts in dairy cows using transrectal ultrasonography. Theriogenology. 52(5): 923-937.

Kaewlamun W., Okuyi M., Humblot P., Techakumphu M. > Ponter A.A. 2011. Does supplementing dairy cows with β-carotene during the dry period affect postpartum ovarian activity, progesterone, and cervical and uterine involution? Theriogenology. 75(6): 1029-1038.

Kesler D.J. > Garverick H.A. 1982. Ovarian cysts in dairy cattle: a review. Journal of Animal Science. 55(5): 1147-1159.

Lopez-Gatius F. > Lopez-Bejar M. 2002. Reproductive performance of dairy cows with ovarian cysts after different GnRH and cloprostenol treatments. Theriogenology. 58(7): 1337-1348.

Lucy M.C., Beck J., Staples C.R., Head H.H., De La Sota R.L. > Thatcher W.W. 1992. Follicular dynamics, plasma metabolites, hormones and insulin-like growth factor I (IGF-I) in lactating cows with positive or negative energy balance during the preovulatory period. Reproduction Nutrition Development. 32: 331-341.

Meyer J.P., Radeliff R.P., Rhoads M.L., Bader J.F., Murphy C.N. > Lucy M.C. 2007. Timed artificial insemination of two consecutive services in dairy cows using prostaglandin F2-alpha and gonadotropin-releasing hormone. Journal of Dairy Science. 90(2): 691-698.

Nakao T., Sugihashi A., Saga N., Tsunoda N. > Kawata K. 1983. Use of milk progesterone enzyme immunoassay for differential diagnosis of follicular cyst, luteal cyst and cystic corpus luteum in cows. American Journal of Veterinary Research. 44: 888-890.

Nessan G.K., King G.J., McKay G.W., Thomson J.D. > Bertrand W. 1977. Treatment of cystic ovarian degeneration in dairy cows with gonadotropic releasing hormone or human chorionic gonadotropic hormone. Canadian Veterinary Journal. 18(2): 33-37.

Peter A.T. 2004. An update on cystic ovarian degeneration in cattle. Reproduction in Domestic Animals. 39(1): 1-7.

Probo M., Comin A., Mollo A., Cairoli F., Stradaioli G. > Veronesi M.C. 2011. Reproductive performance of dairy cows with luteal or follicular ovarian cysts after treatment with buserelin. Animal Reproduction Science. 127(3-4): 135-139.

Santos J.E., Huber J.T., Theurer C.B. Nussio C.B., Nussio L.G., Tarazon M. > Fish D. 2000. Effects of grain processing and bovine somatotropin on metabolism and ovarian activity of dairy cows during early lactation. Journal of Dairy Science. 83(5): 1004-1015.

Schweıgert F.J., Engalbert F., Mothes R., Hurtıenne A. > Immıg I. 2007. Cooperative European study for the validation of a novel cow-side β-carotene assay in serum and blood. In: Program of 13th International Conference on Production Disease in Farm Animals (Leipzig, Germany). p.162.

Sheldon I.M. > Noakes D.E. 1998. Comparison of three treatments for bovine endometritis. Veterinary Record. 142(21): 575-579.

Silvia W.J., Hatler T.B., Nugent A.M. > Laranja da Fonseca L.F. 2002. Ovarian follicular cysts in dairy cows: An abnormality in folliculogenesis. Domestic Animal Endocrinology. 23: 167-177.

Stevenson J.S. > Tiffany S.M. 2004. Resychronizing estrus and ovulation after not- pregnant diagnosis and various ovarian states including cysts. Journal of Dairy Science. 87(11): 3658-3664.

Taponen J., Katila T. > Rodríguez-Martίnez H. 1999. Induction of ovulation with Gonadotropgin-Releasing Hormone during proestrus in cattle: Influence on subsequent follicular growth and luteal function. Animal Reproduction Science. 55(2): 91-105.

Tebble J.E., O’Donnell M.J. > Dobson H. 2001. Ultrasound diagnosis and treatment outcome of cystic ovaries in cattle. Veterinary Record. 148(13): 411-413.

Teshome E., Kebede A., Abdela N. > Ahmed W.M. 2016. Ovarian Cyst and its Economic Impact in Dairy Farms: A Review. Global Veterinaria. 16(5): 461-471.

Thienpont L.M., Verhseghe P.G., Van Brussel K.A. > De Leenheer A.P. 1998. Efforts by industry toward standardization of serum estradiol-17β measurements. Clinical Chemistry. 44(3): 671-674.

Vanholder T., Opsomer G. > De Kruif A. 2006. Aetiology and pathogenesis of cystic ovarian follicles in dairy cattle: a review. Reproduction Nutrition Development. 46(2): 105-119.

Weng B.C., Chew B.P., Wong T.S., Park J.S., Kim H.W. > Lepine A.J. 1999. β-Carotene uptake and changes in ovarian steroids and uterine proteins during the estrous cycle in the canine. Journal of Animal Science. 78(5): 1284-1290.

Wiltbank M.C. > Pursley J.R. 2014. The cow as an induced ovulator: Timed AI after synchronization of ovulation. Theriogenology. 81(1): 170-185.

Yıldız H., Kaygusuzoğlu E. > Kızıl Ö. 2005. Serum progesterone, vitamin A, E, C and β-carotene levels in pregnant and nonpregnant cows post-mating. Journal of Animal and Veterinary Advances. 4(3): 381-384.

Youngquist R.S. 1986. Cystic follicular degeneration in the cow. In: Morrow D.A. (Ed). Current Therapy in Theriogenology. 2nd edn. Philadelphia: WB Saunders Co., pp.243-246.

Published

2018-01-01

How to Cite

Emre, B., Küçükaslan, İbrahim, Ağaoğlu, A. R., Ay, S. S., Kaya, D., Somali, M., & Aslan, S. (2018). The Effects of Separate and Combined Use of PGF2α and GnRH Hormones and the Addition of Βeta-Carotene on Fertility Parameters in Dairy Cows with Ovarian Cysts. Acta Scientiae Veterinariae, 46(1), 9. https://doi.org/10.22456/1679-9216.84205

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